Competition roster
| Start number | Name | Gender | Category | Club | Paid |
|---|---|---|---|---|---|
| 5 | Eliška Blaščáková | W | W40 | Yes | |
| 44 | Petra D. | W | W40 | Čmaňa a Pekáč | Yes |
| 93 | Pavla Fučíková | W | W40 | Yes | |
| 102 | Jana Hladká | W | W40 | Yes | |
| 11 | Barbora Hronová   | W | W40 | Yes | |
| 137 | Monika Kefurtová | W | W40 | Yes | |
| 48 | Kristina M. | W | W40 | Výzva 2021 | Yes |
| 138 | Kristýna Pašková | W | W40 | Yes | |
| 171 | Marie Prokopiusová | W | W40 | Yes | |
| 20 | Jana S.   | W | W40 | Litoměřice | Yes |
| 38 | Andrea Slaninová | W | W40 | Yes | |
| 143 | Ivana Staňková | W | W40 | adidas Runners Prague | Yes |
| 28 | Majka Tykalová  | W | W40 | Báječné ženy v běhu | Yes |
| 12 | Martina Vrbická | W | W40 | Šneci v běhu | Yes |
| 62 | Lucie Wutzelová | W | W40 | Wutzeláčci | Yes |
| 132 | Věra Záhorová | W | W40 | Yes |