Competition roster
| Start number | Name | Gender | Category | Club | Paid |
|---|---|---|---|---|---|
| 107 | Tereza Čejková | W | W30 | Yes | |
| 89 | Kateřina Fišerová   | W | W30 | Yes | |
| 106 | Tereza Fousová  | W | W30 | Yes | |
| 152 | Kateřina Hanychová | W | W30 | Yes | |
| 99 | Lenka Haringerová | W | W30 | Yes | |
| 61 | Barbora Hladíková | W | W30 | Yes | |
| 88 | Libuše Horská | W | W30 | Yes | |
| 56 | Michaela Kosaková    | W | W30 | Šnek v tempu | Yes |
| 1 | Tereza Kovaříková  | W | W30 | RUN Magazine Team | Yes |
| 133 | Zlata Kožaková | W | W30 | Yes | |
| 103 | Andrea Laušová | W | W30 | Yes | |
| 154 | Darina Machalová | W | W30 | Yes | |
| 26 | Aidana Omarova | W | W30 | Yes | |
| 155 | Kateřina Oppelová | W | W30 | Mixouši | Yes |
| 83 | Miroslava Preňková | W | W30 | Rychlonožka | Yes |
| 65 | Monika Zavřelová | W | W30 | Yes |